ms2 dna encoding a single chain dimer of the ms2 coat protein Search Results


synthetic gene fragment encoding a single chain dimer of the ms2 protein  (Thermo Fisher)
90
Thermo Fisher synthetic gene fragment encoding a single chain dimer of the ms2 protein
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Synthetic Gene Fragment Encoding A Single Chain Dimer Of The Ms2 Protein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synthetic gene fragment encoding a single chain dimer of the ms2 protein/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
synthetic gene fragment encoding a single chain dimer of the ms2 protein - by Bioz Stars, 2026-03
90/100 stars
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pms2 dimer  (Thermo Fisher)
86
Thermo Fisher pms2 dimer
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Pms2 Dimer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pms2 dimer/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
pms2 dimer - by Bioz Stars, 2026-03
86/100 stars
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ms2 coat protein (mcp) coding sequence  (Addgene inc)
90
Addgene inc ms2 coat protein (mcp) coding sequence
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Ms2 Coat Protein (Mcp) Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ms2 coat protein (mcp) coding sequence/product/Addgene inc
Average 90 stars, based on 1 article reviews
ms2 coat protein (mcp) coding sequence - by Bioz Stars, 2026-03
90/100 stars
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ms2 dna encoding a single chain dimer of the ms2 coat protein  (GenScript corporation)
90
GenScript corporation ms2 dna encoding a single chain dimer of the ms2 coat protein
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Ms2 Dna Encoding A Single Chain Dimer Of The Ms2 Coat Protein, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ms2 dna encoding a single chain dimer of the ms2 coat protein/product/GenScript corporation
Average 90 stars, based on 1 article reviews
ms2 dna encoding a single chain dimer of the ms2 coat protein - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
avitag  (GenScript corporation)
90
GenScript corporation avitag
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Avitag, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avitag/product/GenScript corporation
Average 90 stars, based on 1 article reviews
avitag - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pet-28b  (GenScript corporation)
90
GenScript corporation pet-28b
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Pet 28b, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet-28b/product/GenScript corporation
Average 90 stars, based on 1 article reviews
pet-28b - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
phmm vector  (Addgene inc)
92
Addgene inc phmm vector
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Phmm Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phmm vector/product/Addgene inc
Average 92 stars, based on 1 article reviews
phmm vector - by Bioz Stars, 2026-03
92/100 stars
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rabv cvs n2c deltag mcherry plasmid  (Addgene inc)
93
Addgene inc rabv cvs n2c deltag mcherry plasmid
Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include <t>MS2</t> phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.
Rabv Cvs N2c Deltag Mcherry Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabv cvs n2c deltag mcherry plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
rabv cvs n2c deltag mcherry plasmid - by Bioz Stars, 2026-03
93/100 stars
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Image Search Results


Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include MS2 phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.

Journal: Frontiers in Genetics

Article Title: Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

doi: 10.3389/fgene.2019.00365

Figure Lengend Snippet: Modifying DSB repair pathway choice by association of Cas9 with CtIP. (A) Cas9 induced double-strand breaks are either repaired by HDR or the prevailing NHEJ pathway. DSB repair pathway choice is largely determined by the competition between 53BP1 and BRCA1, triggering either end protection or the CtIP induced resection of DSB ends, followed by the engagement of the NHEJ or HDR pathway. (B) Use of a Cas9-CtIP fusion protein for shifting DSB repair choice toward HDR. (C) Association of Cas9 and CtIP via sgRNA loops that include MS2 phage aptamer sequences bound by fusion proteins of CtIP and the MS2 coat protein. (D) Association of a Cas9-SunTag fusion protein with multiple copies of a SunLigand-CtIP fusion protein.

Article Snippet: Alternatively a 875 bp synthetic gene fragment from pMS2-dimer (Thermo Scientific) encoding a single chain dimer of the MS2 protein ( ) was cloned into the PacI site of pU6Rosa(MS2)-CAG-CtIP(T847E)-EF1-BFP to obtain the pU6Rosa(MS2)-CAG-MS2 di -CtIP(T847E)-EF1-BFP vector.

Techniques:

DSB repair modification by MS2-CtIP and SunL-CtIP fusion proteins in HEK TLR6 reporter cells. (A) Expression vectors for Cas9 or Cas9-SunTag, sgRosa26(MS2), pTLR-donor and CtIP, MS2-CtIP, MS2 di -CtIP, or SunL-CtIP were cotransfected into HEK TLR6 cells and the frequency of Venus + cells (green columns) and RFP + cells (red columns), indicating DSB repair by HDR or NHEJ, was determined by flow cytometry (FACS) 72 h after transfection. (B) Transfection results are shown as bar graphs representing mean values with standard deviation of triplicate samples. Plasmids selected (+) for individual transfection samples are indicated in the table below. Mean values were used to calculate the ratio of Venus + to RFP + cells and p -values ( T -test) to determine the significance in levels of Venus + or RFP + cells between samples 2 and 3–6 (table bottom) and to compare samples 4 and 5 (top horizontal lines). n.s., not significant, p > 0.05. Two independent replicates of the assay were performed that confirmed the results.

Journal: Frontiers in Genetics

Article Title: Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

doi: 10.3389/fgene.2019.00365

Figure Lengend Snippet: DSB repair modification by MS2-CtIP and SunL-CtIP fusion proteins in HEK TLR6 reporter cells. (A) Expression vectors for Cas9 or Cas9-SunTag, sgRosa26(MS2), pTLR-donor and CtIP, MS2-CtIP, MS2 di -CtIP, or SunL-CtIP were cotransfected into HEK TLR6 cells and the frequency of Venus + cells (green columns) and RFP + cells (red columns), indicating DSB repair by HDR or NHEJ, was determined by flow cytometry (FACS) 72 h after transfection. (B) Transfection results are shown as bar graphs representing mean values with standard deviation of triplicate samples. Plasmids selected (+) for individual transfection samples are indicated in the table below. Mean values were used to calculate the ratio of Venus + to RFP + cells and p -values ( T -test) to determine the significance in levels of Venus + or RFP + cells between samples 2 and 3–6 (table bottom) and to compare samples 4 and 5 (top horizontal lines). n.s., not significant, p > 0.05. Two independent replicates of the assay were performed that confirmed the results.

Article Snippet: Alternatively a 875 bp synthetic gene fragment from pMS2-dimer (Thermo Scientific) encoding a single chain dimer of the MS2 protein ( ) was cloned into the PacI site of pU6Rosa(MS2)-CAG-CtIP(T847E)-EF1-BFP to obtain the pU6Rosa(MS2)-CAG-MS2 di -CtIP(T847E)-EF1-BFP vector.

Techniques: Modification, Expressing, Flow Cytometry, Transfection, Standard Deviation

Comparison of Cas9-CtIP, MS2 di -CtIP, and SunL-CtIP fusion proteins. (A) Expression vectors for Cas9, Cas9-CtIP, or Cas9-SunTag, sgRosa26(MS2), pTLR-donor, and MS2 di -CtIP or SunL-CtIP were cotransfected into HEK TLR6 cells and the frequency of Venus + cells (green columns) and RFP + cells (red columns), indicating DSB repair by HDR or NHEJ, was determined by FACS analysis 72 h after transfection. (B) Transfection results are shown as bar graphs representing mean values ± standard deviation of triplicate samples. Plasmids selected (+) for individual transfections are indicated as in the table below. Mean values were used to calculate the ratio of Venus + to RFP + cells and p -values ( T- test) to determine the significance in levels of Venus + or RFP + cells between samples 5 and 3 or 4 (top horizontal lines). Two independent replicates of the assay were performed that confirmed the results.

Journal: Frontiers in Genetics

Article Title: Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

doi: 10.3389/fgene.2019.00365

Figure Lengend Snippet: Comparison of Cas9-CtIP, MS2 di -CtIP, and SunL-CtIP fusion proteins. (A) Expression vectors for Cas9, Cas9-CtIP, or Cas9-SunTag, sgRosa26(MS2), pTLR-donor, and MS2 di -CtIP or SunL-CtIP were cotransfected into HEK TLR6 cells and the frequency of Venus + cells (green columns) and RFP + cells (red columns), indicating DSB repair by HDR or NHEJ, was determined by FACS analysis 72 h after transfection. (B) Transfection results are shown as bar graphs representing mean values ± standard deviation of triplicate samples. Plasmids selected (+) for individual transfections are indicated as in the table below. Mean values were used to calculate the ratio of Venus + to RFP + cells and p -values ( T- test) to determine the significance in levels of Venus + or RFP + cells between samples 5 and 3 or 4 (top horizontal lines). Two independent replicates of the assay were performed that confirmed the results.

Article Snippet: Alternatively a 875 bp synthetic gene fragment from pMS2-dimer (Thermo Scientific) encoding a single chain dimer of the MS2 protein ( ) was cloned into the PacI site of pU6Rosa(MS2)-CAG-CtIP(T847E)-EF1-BFP to obtain the pU6Rosa(MS2)-CAG-MS2 di -CtIP(T847E)-EF1-BFP vector.

Techniques: Expressing, Transfection, Standard Deviation

Oligodeoxynucleotides.

Journal: Frontiers in Genetics

Article Title: Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

doi: 10.3389/fgene.2019.00365

Figure Lengend Snippet: Oligodeoxynucleotides.

Article Snippet: Alternatively a 875 bp synthetic gene fragment from pMS2-dimer (Thermo Scientific) encoding a single chain dimer of the MS2 protein ( ) was cloned into the PacI site of pU6Rosa(MS2)-CAG-CtIP(T847E)-EF1-BFP to obtain the pU6Rosa(MS2)-CAG-MS2 di -CtIP(T847E)-EF1-BFP vector.

Techniques: Sequencing